

In this context, we present a systematic review that organizes and discusses laboratory procedures for SARS-CoV-2 RNA quantification from a wastewater matrix, along with modeling techniques applied to the development of WBE for COVID-19 surveillance.

This tool demands both in-lab and computational work to be meaningful for, among other purposes, the prediction of outbreaks. Wastewater-based epidemiology (WBE) has been able to monitor the circulation of viral pathogens in the population. This virus is predominantly transmitted via respiratory droplets and shed via sputum, saliva, urine, and stool.

On March 11, 2020, the World Health Organization declared the coronavirus disease 2019 (COVID-19), whose causative agent is the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), a pandemic.
